Device

Part:BBa_K598017

Designed by: Qingyang XIAO   Group: iGEM11_Peking_R   (2011-09-27)


pBAD+Adenine Hammerhead Ribozyme+RBS+E0040+B0015

This is an adenine responced up-regulat hammerhead ribozyme followed by a GFP coading sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 919


[edit]
Categories
Parameters
biology an original adenine-upregulate hammerhead ribozyme
familyhammerhead ribozyme, e.g. TPP responsive hammerhead ribozyme
functioneGFP as a reporter gene whose expression responses to various concentration of adenine
ligandsadenine
n/apBAD+Adenine Hammerhead Ribozyme+RBS+E0040+B0015
rbsstrong